I tend to play 4 or 5 "characters" in an MMO - 4 ladies, and, if I get the room, 1 dude - blame Dofus, my first MMO - the men originally looked flat out dumb, and the ladies looked kickass, so I played ladies mostly. Each Dofus race tends towards 3 or 4 play styles, and I used each character to play a different style, depending on how I wanted to play at the time.
I have, since then, played re-interpreted versions of those 5 in every subsequent MMO, doing my best to maintain those play styles, and switching as usual. For example, my current hero profile is "Sollarium" - Originally an Ecaflip's Luck. Major aspects of that class are high risk, high rewards maneuvers, with a strong tool chest of secondary abilities. For me, Sol is a very free spirited wanderer who emphasizes buffs and debuffs, movement/cc abilities, and played up the "luck" aspect of the class. In this game, luck is Charisma, so she's an Uno this time around. The fact that Uno powers are a movement buff and a group buff also "fit" - as does the bard/illusionist emphasis on the non-damage tool kit - and her personality is still intact - including her following the luck god, despite that god being "human." An easy translation, as it were.
Similarly, Bloody Ash was a Sacrier's Blood - a class that grew powerful on taking damage - An Infernal in this game, obviously XD How well an infernal tank that emphasizes Agility and Constitution is going to do, we'll see, but it will be interesting.
And so on.
Because I've had such a long history with these characters, I can't really see playing them as different genders than what they "are." Feels a bit like forcing a friend to change genders for your convenience - ie, not right at all.